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R2C 大鼠gaowan間質細胞瘤

簡要描述：CCL-97 R2C 大鼠gaowan間質細胞瘤，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件

  • 產品型號：CCL-97
  • 廠商性質：生產廠家
  • 更新時間：2024-08-29
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CCL-97 R2C 大鼠gaowan間質細胞瘤，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和優(yōu)培養(yǎng)條件


CCL-97 R2C 大鼠gaowan間質細胞瘤

ATCC® Number: CCL-97™    Price: $438.00
Designations: R2C

Depositors: G Sato

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus （rat）

Morphology:epithelial


Source:Strain: WFu
Organ: testis
Disease: Leydig cell tumor

Cellular Products:steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications:transfection host （Roche FuGENE® Transfection Reagents）

Virus Resistance:poliovirus 2

Reverse Transcript:positive

Age: 2 months

Gender: male

Comments:Stimulation by other hormones or cAMP is not required for hormone secretion.

Propagation: ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture （Ham）. To make the complete growth medium, add the following components to the base medium:

  • fetal bovine serum to a final concentration of 2.5%

  • horse serum to a final concentration of 15%


Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Subcultur



 cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022

ATCC® Number: CCL-97™ Price: $438.00

Designations: R2C

Depositors: G Sato

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus （rat）

Morphology: epithelial



Source: Strain: WFu

Organ: testis

Disease: Leydig cell tumor

Cellular Products: steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host （Roche FuGENE® Transfection Reagents）

Virus Resistance: poliovirus 2

Reverse Transcript: positive

Age: 2 months

Gender: male

Comments: Stimulation by other hormones or cAMP is not required for  detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040

References: 1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022




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